Citation
Analysis of Diphenhydramine (DPH) in Aqueous Solutions

Material Information

Title:
Analysis of Diphenhydramine (DPH) in Aqueous Solutions
Creator:
Kaylandra Woodside
Shokouh Haddadi
Physical Description:
Research summary

Subjects

Subjects / Keywords:
Diphenhydramine
Genre:
dataset ( sobekcm )

Notes

Abstract:
Diphenhydramine (DPH) is an over-the-counter antihistamine which has been reported to be used as a date-rape-drug in drug facilitated crimes (DFC) due to its sedative properties. Due to the high potency and short half-life of DPH, methods that can detect DPH and its metabolites in urine at low concentrations are required. Dispersive liquid-liquid microextraction (DLLME) is a popular extraction technique within the forensic toxicology community due to its low usage of toxic chemicals and speed. DLLME uses a dispersive solvent and an extractive solvent to remove the analyte from the aqueous solution. A DLLME method was used to extract diphenhydramine and one of its metabolites, N-desmethyl diphenhydramine, from aqueous solutions. Aqueous solutions of DPH and its metabolite were prepared in 5 ng/mL to 2 μg/mL ranges. The extractions were conducted at pH of 12 by adding 50 µL of NaOH and 20 µL of phosphate buffer prior to the solution being spiked by 1 mL of a DLLME solvent mixture of toluene and acetonitrile (13:40). After injection, solutions were shaken for 10 min and centrifuged for 10 min. Solutions were then placed into an ice bath for 30 min, and the top toluene layer of each solution was removed and transferred to clean vials. Toluene was then evaporated and the residues were reconstituted with methanol. A second set of solutions used for direct calibration were prepared ranging from 50 ng/mL to 100 µg/mL. The proposed plan was to analyze the extracted compounds using Gas Chromatography coupled with Mass Spectrometry(GC-MS); however, the GC-MS instrument was out of order. Future work includes analysis of the extracted compounds using GC-MS, testing methods to freeze the aqueous layer of the extraction phase using liquid nitrogen, as well as enhancing extraction recoveries of the analytes by conducting a second extraction on the aqueous solutions.
Acquisition:
Collected for SUNY Oswego Institutional Repository by the online self-submittal tool. Submitted by Kaylandra Woodside.

Record Information

Source Institution:
SUNY Oswego Institutional Repository
Holding Location:
SUNY Oswego Institution
Rights Management:
All applicable rights reserved by the source institution and holding location.

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