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|a The Role of α-actinin in Dictyostelium discoideum Response to Mechanical Stimuli |h [electronic resource]. |
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|a 1 Poster : |b Digital ; |c 222 MB |
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|a Cells can sense various chemical and mechanical stimuli, which cause changes in the actin cytoskeleton leading to directed migration of cells toward or away from these stimuli. Dictyostelium discoideum is a social amoeba that has many homologous genes to higher eukaryotes, making it an ideal model organism for the study of amoeboid-type migration typical of neutrophil and metastatic cancer cells. Disruption of actin cytoskeleton removes the ability of cells to respond to mechanical stimuli, but the reasons are unclear. Actin cytoskeleton is made up of globular actin monomers that polymerize into filaments, actin crosslinkers that link and bundle actin filaments into various conformations, and various regulatory actin-binding proteins. α-actinin is an actin crosslinker that has been previously shown to have mechanosensitive properties. In order to determine the role of α-actinin in the ability of cells to respond to mechanical stimuli, we will test activation of the signal transduction network in cells with and without α-actinin following brief exposure to shear flow as a mechanical stimulus. An mCherry-tagged α-actinin construct was first cloned and successfully expressed in wild-type cells. Following brief exposure to shear flow, α-actinin relocalized from the cytosol to the cell cortex, confirming its ability to respond to mechanical stimuli. Further studies will test the response of the signal transduction network using a fluorescently-tagged biosensor in α-actinin null cells with and without the above α-actinin construct. |
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|a Electronic reproduction. |c SUNY Oswego Institutional Repository, |d 2020. |f (Oswego Digital Library) |n Mode of access: World Wide Web. |n System requirements: Internet connectivity; Web browser software. |
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|a SUNY Oswego Institution. |
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|a Collected for SUNY Oswego Institutional Repository by the online self-submittal tool. Submitted by Aaron Cole. |
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|a Dictyostelium discoideum. |
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|a Oswego Digital Library. |
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|a SUNY Oswego Scholarly and Creative Works. |
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|a Summer 2020 Scholarly and Creative Activities Symposium. |
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|a OswegoDL |c SUNY Oswego Scholarly and Creative Works |
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|u https://digitallibrary.oswego.edu/AA00000234/00001 |y Electronic Resource |
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|a https:/digitallibrary.oswego.edu/content/AA/00/00/02/34/00001/Abstractthmthm.jpg |
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|a SUNY Oswego Scholarly and Creative Works |