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200904n^^^^^^^^xx^^^^^^s^^^^^^^^^^^eng^d |
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|a Characterization of Filamin's Involvement in Response to Mechanical Stimuli |h [electronic resource]. |
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|a Directed migration of cells is vital for a number of processes, including uncoordinated
migration of cancer cells. The social amoeba Dictyostelium discoideum is very similar to
mammalian cells, making it an excellent model organism for studying migration. Cells can
migrate directionally in response to mechanical stimuli, such as shear flow, although how cells sense this type of stimulus is unclear. Previous studies in our lab showed that filamin, which is a protein that cross-links actin cytoskeleton in cells, is involved in the ability of cells to respond to mechanical cues. We have now shown that filamin does not affect response to chemical stimuli, suggesting that filamin’s role is specific to responsiveness to mechanical stimuli. Filamin requires the actin-binding domain (ABD) and the dimerization domain (DD) to work together to ensure proper binding and cross-linking of the actin cytoskeleton. To further understand filamin’s role in sensing physical stimuli, we generated an expression construct for filamin with no ABD. Future studies will test the ability of filamin without ABD to rescue the reduced response of filamin-null cells in a mechanical assay. |
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|a Electronic reproduction. |c SUNY Oswego Institutional Repository, |d 2020. |f (Oswego Digital Library) |n Mode of access: World Wide Web. |n System requirements: Internet connectivity; Web browser software. |
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|a SUNY Oswego Institution. |
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|a Collected for SUNY Oswego Institutional Repository by the online self-submittal tool. Submitted by Sarah Buckler. |
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|a Oswego Digital Library. |
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|a SUNY Oswego Scholarly and Creative Works. |
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|a Summer 2020 Scholarly and Creative Activities Symposium. |
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|a OswegoDL |c SUNY Oswego Scholarly and Creative Works |
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|u https://digitallibrary.oswego.edu/SUOS000904/00001 |y Electronic Resource |
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|a https:/digitallibrary.oswego.edu/content/SU/OS/00/09/04/00001/2020 RISE Symposium posterthm.jpg |
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|a SUNY Oswego Scholarly and Creative Works |